Daisuke Mano¹, Atsushi Ogura¹, Toshiyuki Tanaka²,  Rena Ujiie ²,Kyouitiro Mizutani² ,Hitoshi Sakuragi²,Yoshiaki Suzuki², Hiroshi Ujiie³

¹Graduate School of Science and Engineering, Meiji University, 1-1-1 Higashimita, Tama-ku, Kawasaki-shi, Kanagawa 214-0033, Japan, ² RIKEN, Japan ³Tokyo Rosai Hospital

Artificial dura mater composed of biodegradable polymers is said to have low biocompatibility. In this study, He⁺, Ne⁺, Ar⁺, and Kr⁺ irradiation was performed into the biodegradable artificial duramater at an energy of 150 keV with fluences of 1×10¹³, 1×10¹⁴, and 1×10¹⁵,ions/cm² to improve biocompatibility. Specimens used were poly(L-lactide-co-ε-caprolactone) copolymer (Johnson & Johnson K.K Japan). The beam current density was kept at 0.1 μA/cm². Mouse fibroblast cells (L929) were seeded to the sample. And I applied fibrin glue to the artificial endocranium which was the best condition of the cell adhesive property next and performed the experiment that I used for the endocranium of the rabbit. I took out a sample one month later and made a pathology specimen and evaluated adhesion with the organization. The physical properties of the irradiated surface were evaluated by means of FT-IR-ATR and Raman spectroscopy. Cell attachment was improved remarkably. Heavy ion irradiation is more effective than light ions such as He⁺. According to FT-IR-ATR study, -COO- decreased dramatically and new functional groups were produced (assigned to -OH and -COOH) after the irradiation. In the Raman spectra, new broad peaks appeared from 1000 to 2000 cm^-1 with a fluence of 1×10¹⁵ ions/cm², which is assigned to amorphous carbon. The result of in vivo animal study will also be reported in detail.

 

 

Daisuke Mano*, Graduate School of Science and Engineering, Meiji University, Japan
Shinya Morisaki, Graduate School of Science and Engineering, Meiji University, 
Atsushi Ogura, Graduate School of Science and Engineering, Meiji University, 
Toshiyui Tanaka, RIKEN, 
Kyoichiro Mizutani, RIKEN, 
Hitoshi Sakuragi, RIKEN, 
Yoshiaki Suzuki, RIKEN, 
Hiroshi Ujiie, Tokyo Rosai Hospital, 

*presenting author